Page 46 - COS-FOD2015

Shoji et al.

components analysis (PCA) were applied. The metabolites found were Proprionate, Acetate,
key metabolites were further validated statisti- Alanine, Lactic acid, Tyrosine, Phenylalanine
cally through permutations methods (with and Formate as shown in Table 1. Spectra de-
10000 resampling) to ensure reliability and re- rived from saliva of healthy Malaysian adults
producibility of the results. were dominated by Acetate and Propionate as
shown in Table 1.
Results
There is no significant inter-individual variations
1
A sample H NMR spectrum of unstimulated of the key metabolites were observed among
the healthy Malaysian adults. To determine dif-
saliva from healthy Malaysian adults with sever-
ferences between male and female saliva, 20
al resonances identified is shown in Figure 1
healthy female subjects and 5 healthy male
and 2. Resonance assignments were made
1
subjects were recruited. PCA scores plot of H
using Bruker Topspin software. All results were
NMR spectra derived from saliva of healthy fe-
phased and baselined corrected. We have iden-
male and male control showed there was no
tified a small number of key metabolites that
significant variation of the metabolites between
were present as part of the human saliva
female and male, as shown in Figure 3.
metabolome of healthy Malaysian adults. The

1
Figure 1: Median H NMR spectra derived from saliva of healthy controls (aliphatic region).

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