Page 44 - COS-FOD2015

Compendium of Oral Science Volume 1|2015

Original Article

Detection Of Metabolomic Profile Of Saliva In Healthy

Malaysian Adults

2
*,1
4
Han Hing Lee , Lee Chee Yen , Yoshinobu Shoji 3 ,4 , Chin Hoe Teh .
1 Hospital Sultan Haji Ahmad Shah, Jalan Maran 28000 Temerloh, Pahang, Malaysia.
2 Bundaberg Base Hospital,Bundaberg West, Queensland 4670, Australia.
3 Faculty of Dentistry, University Technology MARA Sg. Buloh Campus, Jalan Hospital, 47000 Sungai Buloh,
Malaysia.
4 Bruker (Malaysia) Sdn Bhd, 303, Block A, Mentari Business Park, 46150 Petaling Jaya, Selangor, Malaysia.

Keywords: NMR, healthy adult, saliva, metabolomics

Abstract

Background: Saliva is a readily accessible biofluid that is important for the overall quality of life, func-
tionally essential in the chewing, swallowing, tasting, regulation mouth flora and prevention of caries. The aim of
this study is to assess the global metabolomic profile of saliva in healthy Malaysian adults. Methods: As a first
step to determining and understanding the metabolomic profile of saliva in healthy Malaysian adults, we have
collected saliva samples of 50 adults and measured the salivary metabolite to establish a profiling metabolite
data, Human Metabolome Database (HMDB). Metabolites concentrations of saliva in healthy subjects were
1
measured by using H NMR spectroscopy. Results: The results showed there was no significant inter-individual
variations of the key metabolites observed among the healthy Malaysian adults and there was no significant
variation of the metabolites between female and male subjects. Conclusion: The metabolomic profile of saliva in
healthy Malaysian adults could be used to establish the metabolomic database and used as a comparison for
future study of the saliva of specific diseases.

Introduction

Metabolomics provide an analysis of changing of metabolism and cardiovascular studies [2-3].
metabolite levels in biological samples [1]. “Genomics and proteomics tell you what might
Metabolomics have been applied to a number happen, but metabolomics tells you what actu-
of important areas, which include the discovery ally did happen.” Said Bill Lasley,University of
of biomarkers as well as mechanistic studies California, Davis. Metabolomics is more sensi-
aimed at discovering metabolites or metabolic tive than genomics and proteomics as the
pathways that regulate cellular and physiologi- metabolome measured is the final downstream
cal processes [1]. For example, metabolomics product of transcription and translation, thus
has been applied to clinical conditions such as closest to the phenotype. The most important,
Crohn’s Disease, Cancer, Genetic inborn errors saliva can be the sample for metabolomics
study and has advantages over other biofluids

such as blood and urine. Saliva collection is
*Correspondence to: Yoshinobu Shoji, Faculty of Dentis-
try, University Technology MARA Sg. Buloh Campus, non-invasive and relatively fast compare to oth-
Jalan Hospital, 47000 Sungai Buloh, Malaysia. er biofluids collection [4-5].
E-mail: shoji@salam.uitm.edu.my
Tel: +603-61266620, Faks: +603-61266103

40